Probing the dynamics of intact cells and nuclear envelope precursor membrane vesicles by deuterium solid state NMR spectroscopy.
作者： Marie Garnier-Lhomme , Axelle Grélard , Richard D. Byrne , Cécile Loudet , Erick J. Dufourc
DOI: 10.1016/J.BBAMEM.2007.06.004
关键词:
摘要: Membrane dynamics is an essential part of many cellular mechanisms such as intracellular trafficking, membrane fusion/fission and mitotic organelle reconstitution. The membranes dependent primarily on their phospholipid cholesterol composition how these molecules are ordered in relation to one another. To determine the physical status whole cells or purified subcellular compartments we have developed a novel application exploiting solid-state 2 H-NMR spectroscopy. We utilise this method probe intact sperm nuclear envelope precursor membranes. show, using mass spectrometry, that either multilamellar small unilamellar vesicles deuterium-labelled palmitoyl-oleoylphosphatidylcholine can be used vesicles, respectively. Using order parameters vesicles. demonstrate more dynamic than due higher levels latter. Our new exploited generic for monitoring cells, various domains compartments.
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