IGF1 promotes resistance to apoptosis in melanoma cells through an increased expression of BCL2, BCL-X(L), and survivin.

摘要: IGF1 plays a key role in the development and growth of multiple tumors prevention apoptosis. In melanoma cells, has been shown to mediate resistance anoikis-induced However, effect on other proapoptotic stimuli never reported. Further, molecular mechanisms by which mediates its prosurvival properties cells remain unknown. Here, we demonstrate that impairs onset tumor necrosis factor–related apoptosis-inducing ligand staurosporine-induced apoptosis expressing either wild-type or oncogenic B-Raf. show inhibits mitochondrial damage occurs during apoptosis, thereby indicating acts at level mitochondria antiapoptotic stimuli. Accordingly, increases mRNA levels protein expression members BCL2 family—BCL2 BCL-X(L)—and inhibitor protein, survivin. their specific silencing small interfering RNA prevents protective IGF1. These findings therefore delineate provide basis for clinical strategies designed neutralize target genes.