Relationships between the Transition of the Physical Phase of Membrane Lipids and Photosynthetic Parameters in Anacystis nidulans and Lettuce and Spinach Chloroplasts.

摘要: The transition of the physical phase lipids in membrane fragments a blue-green alga Anacystis nidulans was studied by spin labeling technique. maximum hyperfine splitting electron resonance spectrum N-oxyl-4', 4'-dimethyloxazolidine derivative 5-ketostearic acid plotted against reciprocal absolute temperature gave discontinuity point that characteristic hydrocarbon region lipids. appeared at approximately 13 or 24 C organisms grown 28 38 C, respectively.The dependence curve chlorophyll fluorescence intact cells, fragments, and extracted cells suspended buffer solution showed yield became near temperatures. These findings suggest thylakoid works as native probe for detection transition.The photosynthetic transport reactions measuring oxidoreductive P700 O(2) evolution. Each Arrhenius plots reaction rates composed two straight lines with break activation energy always lower above than below It is proposed to explain these phenomena involving plastoquinone influenced state lipids.The shift between pigment 1 2 measured transients also temperatures; temperatures almost disappeared. This suggests configurational change related dependent on In chloroplasts lettuce spinach, other hand, there no plot Mg(2+)-induced changes fluorescence.It suggested transitions ESR signal, transport, change, well appearance fluorescence, membranes are all liquid crystalline mixed crystal-solid state.