Retinoic acid-mediated anti-inflammatory responses in equine immune cells stimulated by LPS and allogeneic mesenchymal stem cells.

作者: Leila Abdelhamid , Hayam Hussein , Mostafa Ghanem , Nour Eissa

DOI: 10.1016/J.RVSC.2017.05.006

关键词:

摘要: Retinoic acid (RA), an active metabolite of vitamin A, has shown potential therapeutic immunomodulatory properties. Allogeneic mesenchymal stem cells (MSCs)-based therapy is effective approach to induce tissue healing and regeneration in many equine orthopedic conditions. However, MSCs-based therapies induced inflammatory responses vivo. This study aimed to: 1. Determine the effect RA cell culture treatment on lipopolysaccharides (LPS)- allogeneic MSCs-stimulated peripheral blood mononuclear (PBMCs). 2. stimulated MSCs viability morphology. PBMCs had significant decreases anti-inflammatory cytokines (IL-10, IL-1ra, TGF-β1), increases pro-inflammatory mediators (IL-1β, IL-6, TNF-α, SAA), CD14 MHC II percent positive compared LPS- non-stimulated PBMCs. counterbalanced responses. Moreover, significantly improved morphology MSCs. These findings highlighted complications immuno-modulatory cells. In conclusion, use ameliorate associated inflammation may offer advantages that would require further investigations.

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