CHARACTERIZATION OF CHITIN SYNTHASE AND CHITINASE GENE FAMILIES FROM THE AFRICAN MALARIA MOSQUITO

摘要: Chitin metabolism represents an attractive target site for combating insect pests as growth and development are strictly dependent on precisely toned chitin synthesis degradation this process is absent in humans other vertebrates. However, current understanding the involved enzymes rather limited insects. In study, two synthase genes (AgCHS1 AgCHS2 or AgCHSA AgCHSB), 20 chitinase chitinase-like (groups I-VIII) presumably encoding biosynthesis degradation, respectively, were identified characterized African malaria mosquito, Anopheles gambiae. Immunohistochemistry analysis developmental stageand tissue-dependent transcript profiling by using reverse transcription PCR, real-time quantitative situ hybridization revealed new information these genes. Current synthases extended expression profiles such localization of AgCHS1 transcripts eggs, posterior larval midgut, proteins compound eyes, enzyme pupal inter-segments. Chitinase highly diverse their gene structure, domain organization, tissue-specific patterns. Most expressed several stages. some AgCht8 mainly adult stages, AgCht2 AgCht12 specifically foregut, AgCht13 exclusively midgut. Functional each was conducted chitosan/dsRNA nanoparticle-based RNA interference (RNAi) through feeding. The repression which predominantly carcass initiated from mosquito feeding dsRNA suggests systemic nature RNAi larvae. addition, silencing increased susceptibilities to diflubenzuron, whereas enhanced peritrophic matrix disruption thus calcofluor white dithiothreitol. Furthermore, a non-radioactive method adapted optimized examine activity mosquitoes. By method, diflubenzuron nikkomycin Z show vitro inhibition at high concentration cell free system, no vivo observed. CHARACTERIZATION OF CHITIN SYNTHASE AND CHITINASE GENE FAMILIES FROM THE AFRICAN MALARIA MOSQUITO