MicroRNA-122-5p inhibits cell proliferation, migration and invasion by targeting CCNG1 in pancreatic ductal adenocarcinoma.

摘要: Pancreatic ductal adenocarcinoma (PDAC) is a lethal human malignancy, and previous researches support the contribution of microRNA (miRNA) to cancer progression. MiR-122-5p reported participate in regulation various cancers, while function miR-122-5p PDAC remains unclear. In this study, we investigated precise mechanism involved pathogenesis. The expression levels were detected tissues cell lines by miRNA RT-PCR. effects on proliferation explored MTT assays, colony formation assays flow cytometry assays. ability migration invasion was determined transwell Dual Luciferase reporter assay performed validate direct interaction between its target gene. related molecules cycle, apoptosis epithelial–mesenchymal transition (EMT) examined with qRT-PCR western blot. addition, xenograft mouse models applied explore vivo. underexpressed, CCNG1 highly expressed cells. negatively correlated TNM stage, tumor size lymph node metastasis patients. Overexpression suppressed proliferation, vitro inhibited tumorigenesis Furthermore, miR-122-5p. Upregulated could partially reverse caused Moreover, EMT through downregulation CCNG1. inhibit migration, invasion, downregulating PDAC, suggesting potential therapeutic for PDAC.