Regeneration of somatic embryos from protoplasts isolated from an embryogenic suspension culture of white spruce (Picea glauca).
作者： S. M. Attree , F. Bekkaoui , D. I. Dunstan , L. C. Fowke
DOI: 10.1007/BF00272788
关键词:
摘要: Regeneration of white spruce (Picea glauca) somatic embryos from protoplasts derived an embryogenic suspension culture was accomplished using a medium containing 2 mgl(-1) 2,4-D and 1 6-BAP. Divisions within days led to plating efficiencies in the order 24% after 9 days. A reduction osmoticum, necessary for sustained growth, carried out gradually over 30 Embedding agarose 5 cm petri dishes prior transfer blocks bead type culture, formation as early 23 isolation yielded 5-10% 35 culture.
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