Nuclear and cytoplasmic delivery of lactoferrin in glioma using chitosan nanoparticles: Cellular location dependent-action of lactoferrin.
作者： Salma N. Tammam , Hassan M.E. Azzazy , Alf Lamprecht
DOI: 10.1016/J.EJPB.2018.05.027
关键词:
摘要: Lactoferrin (Lf) exerts anti-cancer effects on glioma, however, the exact mechanism remains unclear. Despite possessing a nuclear localization sequence (NLS), Lf was found to allocate only in cytoplasm of glioma 261. therefore loaded into and cytoplasmic targeted nanoparticles (NPs) determine whether delivery would enhance its effect. Upon treatment with 300 800 µg/mL chitosan NPs, Lf-NPs showed 1.3 2.7 folds increase cell viability, whereas at decreased viability by 0.8 comparison free controls. Results suggest that cytotoxicity is attributable allocation. Nuclear induced proliferation rather than cytotoxicity, indicating mode action location dependent. This calls for caution about general use as an protein.
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