T-cell allorecognition of donor glutathione S-transferase T1 in plasma cell-rich rejection.

摘要: AIM: To investigate the role of glutathione S-transferase T1 donor-specific T lymphocytes in plasma cell-rich rejection liver allografts. METHODS: The study group included 22 transplant patients. Among them, 18 patients were mismatched for (GSTT1) alleles (don+/rec-), and 4 matched (don+/rec+). Seven produced anti-GSTT1 antibodies developed (former de novo immune hepatitis). For detection specific lymphocytes, peripheral blood mononuclear cells collected stored liquid nitrogen. memory cell response was studied by adding to cultures a mix 39 custom-made, 15-mer overlapping peptides, which covered entire GSTT1 amino acid sequence. cellular peptides analyzed flow cytometry using markers CD8, CD4, IL-4 IFNγ. RESULTS: Activation CD8+ with different observed exclusively plasma-cell rich (3 out 7), production and/or IFNγ at rate 1%-4.92% depending on peptides. CD4+ most common not exclusive disease, where 5 7 showed percentages activated from 1.24% 31.34%. Additionally, two without disease but mismatch had that became stimulated some (1.45%-5.18%). Highly unexpected finding double positive CD4+CD8low population highest degree activation mismatch, 3 disease. Unfortunately, represent 1% total number stimulation could be 9 due low gated cells. Cells as controls did show any CONCLUSION: Patients can develop T-cell response, potential this pathogenesis is unknown.