Detection of whole chromosomes in micronuclei of cytokinesis-blocked human lymphocytes by antikinetochore staining and in situ hybridization

摘要: The effect of cytochalasin B (Cyt-B; 3 and 6 micrograms/ml; for the last 28 h) on micronuclei (MN) was studied in 72-h purified lymphocyte cultures three male donors. frequency MN much higher multinucleate cells (mean 100-204 per 1000 cells) than binucleate 8.2-21.0 cells), tetranucleate containing more trinucleate cells. presence whole chromosomes two separate experiments by immunofluorescence using antikinetochore (CREST) serum a centromeric alphoid DNA oligomer probe (in situ hybridization, ISH). In tri- tetra-nucleate produced Cyt-B, were clearly often kinetochore-positive (K+) 82-86%) centromere-positive (C+) 73-83%) mononucleate no Cyt-B 63% CREST 50% ISH), indicating that most excess due to chromosomes. lymphocytes had about as high prevalence K+MN 79-84%) cells, despite their low count. Also ISH analysis, majority positively stained 58-62%). If it is assumed extra labelled are present findings suggest could be responsible approximately 45-57% (CREST data) or 17-23% (ISH cells.(ABSTRACT TRUNCATED AT 250 WORDS)