Ultrafast three-dimensional microbubble imaging in vivo predicts tissue damage volume distributions during nonthermal brain ablation

摘要: Transcranial magnetic resonance imaging (MRI)-guided focused ultrasound (FUS) thermal ablation is under clinical investigation for non-invasive neurosurgery, though its use restricted to central brain targets due primarily skull heating effects. The combination of FUS and contrast agent microbubbles greatly reduces the exposure levels needed ablate tissue may help facilitate transcranial throughout brain. However, sources variability exist during microbubble-mediated procedures that necessitate continued development systems methods online treatment monitoring control, ensure excessive and/or off-target bioeffects are not induced from exposures. Methods: Megahertz-rate three-dimensional (3D) microbubble in vivo was performed nonthermal rabbit using a clinical-scale prototype transmit/receive hemispherical phased array system. Results: In-vivo volumetric acoustic over microsecond timescales uncovered spatiotemporal dynamics hidden by conventional whole-burst temporal averaging. Sonication-aggregate ultrafast 3D source field intensity data were predictive damage volume distributions measured post-treatment MRI confirmed via histopathology. Temporal under-sampling emissions, which common practice field, found impede performance highlighted importance capturing adequate control purposes. Conclusion: capability imaging, reported here first time, will enable future treatments with unparalleled precision accuracy, accelerate translation both body.