Influenza A virus NS1 protein activates the phosphatidylinositol 3-kinase (PI3K)/Akt pathway by direct interaction with the p85 subunit of PI3K.
作者： Yeun-Kyung Shin , Qiang Liu , Suresh K. Tikoo , Lorne A. Babiuk , Yan Zhou
关键词:
摘要: Influenza A virus infection activates the phosphatidylinositol 3-kinase (PI3K)/Akt pathway, but mechanism is not clear. Here, it reported that influenza NS1 protein responsible for PI3K/Akt pathway activation. It was demonstrated interacts with p85 regulatory subunit of PI3K via direct binding to SH3 and C-terminal SH2 domains p85. Consensus motifs were found in NS1, namely an SH2-binding motif (YXXXM) at aa 89, SH3-binding 1 (PXXP) around 164 2 212. Mutant encoding mutations SH-binding failed interact SH did activate pathway. The mutant attenuated Madin-Darby canine kidney cells. Our study has established a novel function NS1: by interacting motifs, can
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