The adhesion of putative probiotic lactobacilli to cultured epithelial cells and porcine intestinal mucus
作者： X.J. Li , L.Y. Yue , X.F. Guan , S.Y. Qiao
DOI: 10.1111/J.1365-2672.2007.03636.X
关键词:
摘要: Aims: To investigate the adhesion of lactobacilli and their subsequent competitive exclusion ability against pathogens. Methods Results: Four species putative probiotic were studied for abilities. First, to Caco-2 cells was examined by light electron microscopy. The four then labelled [methyl-3H] thymidine porcine intestinal mucus determined radioactivity. tested showed best on ileal compared with duodenal jujenal mucus. Oxidative compound pre-treatment (NaIO3 NaIO4) dramatically decreased Pre-treating proteolytic enzymes (proteinase K trypsin) resulted in increase Lactobacillus serotype Reuteri I2021, but results other variable. Fermentum I5007 greatest potential exerted Salmonella Escherichia. Conclusions: Adhesion is species-specific. Lactobacilli higher index have better ability. Significance Impact Study: This study suggests that there a positive correlation between lactobacilli. Additionally, vitro assay feasible way screen unknown lactobacilli, potentially future industrial applications.
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