Genotyping of the Major SARS-CoV-2 Clade by Short-Amplicon High-Resolution Melting (SA-HRM) Analysis.

摘要: The genome of the SARS-CoV-2 virus, causal agent COVID-19 pandemic, has diverged due to multiple mutations since its emergence as a human pathogen in December 2019. Some have defined several clades that seem behave differently terms regional distribution and other biological features. Next-generation sequencing (NGS) approaches are used classify sequence variants viruses from individual patients. However, cost relative scarcity NGS equipment expertise developing countries prevent studies aimed associate specific clinical features outcomes such territories. As March 2021, GR clade derivatives, including B.1.1.7 B.1.1.28 variants, predominate worldwide. We implemented post-PCR small-amplicon high-resolution melting analysis genotype isolated saliva This procedure was able clearly distinguish two groups samples SARS-CoV-2-positive predicted, according their profiles, contain non-GR viruses. grouping validated by means amplification-refractory mutation system (ARMS) assay well Sanger sequencing.