Sensory Neuropeptides and their Receptors Participate in Mechano-Regulation of Murine Macrophages
作者： Dominique Muschter , Anna-Sophie Beiderbeck , Tanja Späth , Christian Kirschneck , Agnes Schröder
DOI: 10.3390/IJMS20030503
关键词:
摘要: This study aimed to analyze if the sensory neuropeptide SP (SP) and neurokinin receptor 1 (NK1R) are involved in macrophage mechano-transduction, similar chondrocytes, alpha-calcitonin gene-related peptide (αCGRP) CGRP (CRLR/Ramp1) show comparable activity. Murine RAW264.7 macrophages were subjected a cyclic stretch for 1–3 days 4 h/day. Loading effects analyzed gene protein expression of neuropeptides their receptors, adhesion, apoptosis, proliferation ROS bone marrow-derived (BMM) isolated after surgical osteoarthritis (OA) induction proliferation, apoptosis osteoclastogenesis response loading. induced NK1R CRLR/Ramp1 altered macrophages. mRNA level decreased loading whereas αCGRP was stabilized. reduced adhesion loaded both initially increased activity followed by time-dependent suppression. OA sensitized BMM caspase 3/7 mediated Both neuropeptides, αCGRP, receptors murine mechano-transduction affecting impact on indicate that OA-associated biomechanical alterations might affect population.
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sci-hub.se 参考文章(63)
Yong Guo, Yang Wang, Yinqin Liu, Haitao Wang, Chun Guo, Xizheng Zhang, Chaoyong Bei, Effect of the same mechanical loading on osteogenesis and osteoclastogenesis in vitro Chinese journal of traumatology. ,vol. 18, pp. 150- 156 ,(2015) , 10.1016/J.CJTEE.2014.09.004
I. Fristad, V. Vandevska-Radunovic, K. Fjeld, S. J. Wimalawansa, I. Hals Kvinnsland, NK1, NK2, NK3 and CGRP1 receptors identified in rat oral soft tissues, and in bone and dental hard tissue cells. Cell and Tissue Research. ,vol. 311, pp. 383- 391 ,(2003) , 10.1007/S00441-002-0691-Z
Craig M. Bullock, Peter Wookey, Andrew Bennett, Ali Mobasheri, Ian Dickerson, Sara Kelly, Peripheral Calcitonin Gene-Related Peptide Receptor Activation and Mechanical Sensitization of the Joint in Rat Models of Osteoarthritis Pain Arthritis & Rheumatism. ,vol. 66, pp. 2188- 2200 ,(2014) , 10.1002/ART.38656
Dominique Muschter, Claudia Göttl, Mandy Vogel, Joachim Grifka, Rainer H. Straub, Susanne Grässel, Reactivity of rat bone marrow-derived macrophages to neurotransmitter stimulation in the context of collagen II-induced arthritis Arthritis Research & Therapy. ,vol. 17, pp. 169- 169 ,(2015) , 10.1186/S13075-015-0684-4
N. Yamamoto, K. Fukuda, T. Matsushita, M. Matsukawa, F. Hara, C. Hamanishi, Cyclic tensile stretch stimulates the release of reactive oxygen species from osteoblast-like cells. Calcified Tissue International. ,vol. 76, pp. 433- 438 ,(2005) , 10.1007/S00223-004-1188-4
A. Kadow-Romacker, J.E. Hoffmann, G. Duda, B. Wildemann, G. Schmidmaier, Effect of mechanical stimulation on osteoblast- and osteoclast-like cells in vitro. Cells Tissues Organs. ,vol. 190, pp. 61- 68 ,(2009) , 10.1159/000178022
Stefan Fernandez, Melissa A. Knopf, Sasha K. Bjork, Joseph P. McGillis, Bone marrow-derived macrophages express functional CGRP receptors and respond to CGRP by increasing transcription of c-fos and IL-6 mRNA. Cellular Immunology. ,vol. 209, pp. 140- 148 ,(2001) , 10.1006/CIMM.2001.1795
S. J. Millward-Sadler, A. Mackenzie, M. O. Wright, H.-S. Lee, K. Elliot, L. Gerrard, C. E. Fiskerstrand, D. M. Salter, J. P. Quinn, Tachykinin expression in cartilage and function in human articular chondrocyte mechanotransduction. Arthritis & Rheumatism. ,vol. 48, pp. 146- 156 ,(2003) , 10.1002/ART.10711
Philip Hasbak, Anette Sams, Søren Schifter, Jenny Longmore, Lars Edvinsson, CGRP receptors mediating CGRP-, adrenomedullin- and amylin-induced relaxation in porcine coronary arteries. Characterization with ‘Compound 1' (WO98/11128), a non-peptide antagonist British Journal of Pharmacology. ,vol. 133, pp. 1405- 1413 ,(2001) , 10.1038/SJ.BJP.0704210
Alexandra Berger, Anne H. Tran, Christopher J. Paige, Co-regulated decrease of Neurokinin-1 receptor and Hemokinin-1 gene expression in monocytes and macrophages after activation with pro-inflammatory cytokines. Journal of Neuroimmunology. ,vol. 187, pp. 83- 93 ,(2007) , 10.1016/J.JNEUROIM.2007.04.019