Effect of the same mechanical loading on osteogenesis and osteoclastogenesis in vitro
作者： Yong Guo , Yang Wang , Yinqin Liu , Haitao Wang , Chun Guo
DOI: 10.1016/J.CJTEE.2014.09.004
关键词: Cell biology 、 Mesenchymal stem cell 、 Acid phosphatase 、 Cellular differentiation 、 Chemistry 、 Osteocalcin 、 Matrix (biology) 、 Alkaline phosphatase 、 Tartrate-resistant acid phosphatase 、 Osteoclast
摘要: Purpose: To investigate the influence of same mechanical loading on osteogenesis and osteoclastogenesis in vitro. Methods: Primary osteoblasts, bone marrow-derived mesenchymal stem cells (BMSCs, cultured osteoinductive medium) RAW264.7 osteoclast inductive medium were all subjected to a 1000 us at 1 Hz cyclic stretch for 30 min (twice day). Results: After stimulation, alkaline phosphatase (ALP) activity, osteocalcin protein level osteoblasts BMSCs enhanced, mRNA levels ALP collagen type I increased. Additionally, extracellular-deposited calcium both At time, the activity secreted tartrate-resistant acid phosphatase, number phosphatase-positive multinucleated cells, matrix metalloproteinase-9 extracellular solvency decreased. Conclusion: The results demonstrated that enhanced promoted osteoblastic differentiation restrained vitro.
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