Cellular Capacities for High-Light Acclimation and Changing Lipid Profiles across Life Cycle Stages of the Green Alga Haematococcus pluvialis

摘要: The unicellular microalga Haematococcus pluvialis has emerged as a promising biomass feedstock for the ketocarotenoid astaxanthin and neutral lipid triacylglycerol. Motile flagellates, resting palmella cells, cysts are major life cycle stages of H. pluvialis. Fast-growing motile cells usually used to induce triacylglycerol biosynthesis under stress conditions (high light or nutrient starvation); however, productivity bioproducts compromised due susceptibility stress. This study revealed that Photosystem II (PSII) reaction center D1 protein, manganese-stabilizing protein PsbO, several membrane glycerolipids (particularly chloroplast lipids monogalactosyldiacylglycerol phosphatidylglycerol), decreased dramatically in high (HL). In contrast, which transformed from after an extended period time favorable growth conditions, have developed multiple protective mechanisms—including reduction content, downplay linear photosynthetic electron transport, activating nonphotochemical quenching mechanisms—while accumulating Consequently, PSII proteins (D1 PsbO) remained relatively stable subjected HL. Introducing instead may greatly increase production culture.