Resorbable polyesters in cartilage engineering: Affinity and biocompatibility of polymer fiber structures to chondrocytes
作者： M. Sittinger , D. Reitzel , M. Dauner , H. Hierlemann , C. Hammer
DOI: 10.1002/(SICI)1097-4636(199622)33:2<57::AID-JBM1>3.0.CO;2-K
关键词:
摘要: The resorbable polymers polyglycolic acid (PGA) and polylactic (PLA) are gaining increasing importance in tissue engineering cell transplantation. present investigation was focused on the biocompatibility retaining behavior of PGA/poly-L-lactide (PLLA) (90/10) PLLA nonwoven structures for vitro development chondrocyte-polymer constructs. effect relevant monomers to chondrocytes analyzed. Type II collagen poly-L-lysine were compared improve loading PGA/PLLA polymer nonwovens with chondrocytes. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) test applied quantifications. At concentrations above 2 mg/mL, glycolic more cytotoxic than lactic acid. As shown by pH equilibration, is not due merely acidity α-hydroxy acids. Regarding degradation products, acid, L(+) biocompatible polyglycolide. Collagen type generally improved seeding engineering; however, their efficiency varies depending fiber structure. © 1996 John Wiley & Sons, Inc.
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