Long-term preservation of bone marrow and stem cell pool in dogs.

摘要: A canine model in vivo was established to assess the viability of stem cells frozen and stored for prolonged periods liquid nitrogen. Forty-six foxhounds received total-body irradiation (1000 rads at 9 rads/min) followed by infusion autologous fresh bone marrow or 2 5 mo vapor phase nitrogen (-140°C). The results demonstrated a direct relationship between dose infused, percentage successful engraftments, kinetics peripheral recovery. minimum engraftment 0.1 0.25 × 108 nucleated cells/kg. There no difference (100% recovery cells). Following 5-mo storage period, suggested slight but not statistically significant decrease cell viability. If it could be that this technique successfully preserves human cells, these data would support inclusion rescue management patients with malignant diseases as an adjunct ablative chemotherapy.