Microsomal Glucosidases of Rat Liver. Partial Purification and Inhibition by Disaccharides
作者： Rodolfo A. UGALDE , Roberto J. STANELONI , Luis F. LELOIR
DOI: 10.1111/J.1432-1033.1980.TB06144.X
关键词:
摘要: Further work on microsomal glucosidases of rat liver has confirmed that at least two enzymes are involved in the removal glucose from glucose-containing oligosaccharide. One acts oligosaccharide containing three residues and another which one or glucoses. The glucosidase (Glc)2(Man)9(GlcNAc)2 could be purified with a ConcanavalinA—Sepharose column followed by electrofocusing. This preparation was active glucoses. Heat inactivation inhibition disaccharides parallel for both activities. Inhibition (Glc)3(Man)9(GlcNAc)2 obtained kojibiose an α 1–2 linkage, while acting (Glc)1–2(Man)9-(GlcNAc)2 inhibited nigerose (α 1–3 linkage), maltose 1–4 linkage) higher concentration. None β anomers inhibited. These results consistent configuration glucoses dolichyl-dinhosphate-linked Kojibiose found to inhibit action not only free but also protein-bound one.
sci-hub.se 参考文章(16)
R.G. Spiro, M.J. Spiro, V.D. Bhoyroo, Processing of carbohydrate units of glycoproteins. Characterization of a thyroid glucosidase. Journal of Biological Chemistry. ,vol. 254, pp. 7659- 7667 ,(1979) , 10.1016/S0021-9258(18)35997-0
A Herscovics, B Bugge, R W Jeanloz, Glucosyltransferase activity in calf pancreas microsomes. Formation of dolichyl D[14C]glucosyl phosphate and 14C-labeled lipid-linked oligosaccharides from UDP-D-[14C]glucose. Journal of Biological Chemistry. ,vol. 252, pp. 2271- 2277 ,(1977) , 10.1016/S0021-9258(17)40550-3
Stuart Kornfeld, E. Li, I. Tabas, The synthesis of complex-type oligosaccharides. II. Characterization of the processing intermediates in the synthesis of the complex oligosaccharide units of the vesicular stomatitis virus G protein. Journal of Biological Chemistry. ,vol. 253, pp. 7771- 7778 ,(1978) , 10.1016/S0021-9258(17)34436-8
P W Robbins, S J Turco, The initial stages of processing of protein-bound oligosaccharides in vitro. Journal of Biological Chemistry. ,vol. 254, pp. 4560- 4567 ,(1979) , 10.1016/S0021-9258(17)30048-0
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
Roberto J. STANELONI, Rodolfo A. UGALDE, Luis F. LELOIR, Addition of glucose to dolichyl diphosphate oligosaccharide and transfer to protein. FEBS Journal. ,vol. 105, pp. 275- 278 ,(1980) , 10.1111/J.1432-1033.1980.TB04498.X
R.J. Staneloni, L.F. Leloir, The biosynthetic pathway of the asparagine-linked oligosaccharides of glycoproteins Trends in Biochemical Sciences. ,vol. 4, pp. 65- 67 ,(1979) , 10.1016/0968-0004(79)90265-2
J.Mark Michael, Stuart Kornfeld, Partial purification and characterization of the glucosidases involved in the processing of asparagine-linked oligosaccharides Archives of Biochemistry and Biophysics. ,vol. 199, pp. 249- 258 ,(1980) , 10.1016/0003-9861(80)90278-7
R. J. Staneloni, L. F. Leloir, Oligosaccharides containing glucose and mannose in glycoproteins of the thyroid gland Proceedings of the National Academy of Sciences of the United States of America. ,vol. 75, pp. 1162- 1166 ,(1978) , 10.1073/PNAS.75.3.1162
LF Leloir, NH Behrens, Dolichol Monophosphate Glucose: An Intermediate in Glucose Transfer in Liver Proceedings of the National Academy of Sciences of the United States of America. ,vol. 66, pp. 153- 159 ,(1970) , 10.1073/PNAS.66.1.153