Differential expression of multiple cathepsin mRNAs in the rat testis during maturation and following lonidamine induced tissue restructuring

摘要: In the seminiferous epithelium, germ cell development behind blood-testis barrier involves continual degradation and renewal of inter-testicular junctions. This allows: (i) translocation developing cells from basal lamina to adluminal compartment during spermatogenesis, (ii) eventual release mature spermatids into tubular lumen spermiation. Throughout cellular debris must also be removed epithelium. Thus, it is conceivable that proteases, protease inhibitors, junctional components are involved in these events. The present study sought examine whether testicular can express multiple cathepsin mRNAs given proteases processing proteins as well tissue regeneration. By using total RNA isolated primary cultures Sertoli, Leydig, for reverse-transcription polymerase chain reaction (RT-PCR), B, C, D, H, L, S were shown expressed by Sertoli Leydig cells, whereas adult rats all above except D. postnatal maturation, steady-state mRNA levels remain relatively unchanged with exception H whose level increased maturation peaked at 45-60 days age. Using lonidamine, an anti-spermatogenic drug which known induce premature without affecting function disrupting inter-Sertoli-germ junctions, we have examined differential expression testis time extensive restructuring. It was noted L significantly concomitant disappearance elongate when most round spermatocytes depleted. These results illustrate intricate inter-relationship between