Ligase chain reaction for detection of Neisseria gonorrhoeae in urogenital swabs.

摘要: The ligase chain reaction (LCR) is an in vitro nucleic acid amplification technique that exponentially amplifies targeted DNA sequences. In a multicenter study, we evaluated the use of 4-h LCR-based assay for diagnosis Neisseria gonorrhoeae infection cervix and male urethra. LCR results were compared with those culture N. by using selective media. This target sequences within opacity gene. Discordant LCR-positive culture-negative specimens further testing another which used gonorrhoeae-specific pilin probe sets. A total 1,539 female endocervical 808 urethral swab study. An expanded "gold standard" was defined to include all culture-positive as well culture-negative, confirmed specimens. After resolution discrepant samples, sensitivities assays 97.3 98.5%, respectively, specificities 99.6 99.8%, respectively. Resolved 83.9 96.5% specimens, gonorrhea rapid, highly sensitive nonculture method detecting gonococcal