Cell migration within confined sandwich-like nanoenvironments.
作者： José Ballester-Beltrán , Myriam Lebourg , Patricia Rico , Manuel Salmerón-Sánchez
DOI: 10.2217/NNM.14.217
关键词:
摘要: Aim: We introduced sandwich-like culture as a tool to engineer the cellular nanoenvironment by tuning protein presentation and activation of dorsal ventral receptors. aim at studying cell migration under more similar conditions 3D physiological one. Materials & methods: have investigated different nanoenvironments changing coating using materials that adsorb proteins in conformation, seeking show their specific role migration. Results: Cell within sandwich cultures greatly differs from 2D cultures, shares some similarities with environments is highly dependent on nanoenvironment. Beyond differences morphology migration, stimulation promotes remodeling extracellular matrix over simple receptor traditional cultures. Conclusion: Local(nano) receptors alter comparison standa...
core.ac.uk
elsevier.com
futuremedicine.com
sci-hub.se 参考文章(56)
Stefan Hüttelmaier, John S. Condeelis, Erwin P. Bottinger, Jeffrey E. Segall, Yuri Oleynikov, Robert H. Singer, Weigang Wang, Victoria Centonze Frohlich, John G. White, Jiri Zavadil, Lukas Cermak, Jeffrey B. Wyckoff, Single cell behavior in metastatic primary mammary tumors correlated with gene expression patterns revealed by molecular profiling. Cancer Research. ,vol. 62, pp. 6278- 6288 ,(2002)
Richard O. Hynes, Integrins: Bidirectional, Allosteric Signaling Machines Cell. ,vol. 110, pp. 673- 687 ,(2002) , 10.1016/S0092-8674(02)00971-6
I E Collier, S M Wilhelm, A Z Eisen, B L Marmer, G A Grant, J L Seltzer, A Kronberger, C S He, E A Bauer, G I Goldberg, H-ras oncogene-transformed human bronchial epithelial cells (TBE-1) secrete a single metalloprotease capable of degrading basement membrane collagen. Journal of Biological Chemistry. ,vol. 263, pp. 6579- 6587 ,(1988) , 10.1016/S0021-9258(18)68680-6
Shuh Narumiya, Toshimasa Ishizaki, Masayoshi Ufhata, Use and properties of ROCK-specific inhibitor Y-27632. Methods in Enzymology. ,vol. 325, pp. 273- 284 ,(2000) , 10.1016/S0076-6879(00)25449-9
José Ballester-Beltrán, Myriam Lebourg, Manuel Salmerón-Sánchez, Dorsal and ventral stimuli in sandwich-like microenvironments. Effect on cell differentiation. Biotechnology and Bioengineering. ,vol. 110, pp. 3048- 3058 ,(2013) , 10.1002/BIT.24972
Douglas A Lauffenburger, Alan F Horwitz, Cell Migration: A Physically Integrated Molecular Process Cell. ,vol. 84, pp. 359- 369 ,(1996) , 10.1016/S0092-8674(00)81280-5
Chris S Hughes, Lynne M Postovit, Gilles A Lajoie, None, Matrigel: A complex protein mixture required for optimal growth of cell culture Proteomics. ,vol. 10, pp. 1886- 1890 ,(2010) , 10.1002/PMIC.200900758
Markus Glaß, Birgit Möller, Anne Zirkel, Kristin Wächter, Stefan Hüttelmaier, Stefan Posch, Cell migration analysis: Segmenting scratch assay images with level sets and support vector machines Pattern Recognition. ,vol. 45, pp. 3154- 3165 ,(2012) , 10.1016/J.PATCOG.2012.03.001
S. L. Schor, I. R. Ellis, K. Harada, K. Motegi, A. R. A. Anderson, M. A. J. Chaplain, R. P. Keatch, A. M. Schor, A Novel ''Sandwich'' Assay for Quantifying Chemo-Regulated Cell Migration Within 3-Dimensional Matrices: Wound Healing Cytokines Exhibit Distinct Motogenic Activities Compared to the Transmembrane Assay Cytoskeleton. ,vol. 63, pp. 287- 300 ,(2006) , 10.1002/CM.20123
Stephanie I. Fraley, Yunfeng Feng, Ranjini Krishnamurthy, Dong-Hwee Kim, Alfredo Celedon, Gregory D. Longmore, Denis Wirtz, A distinctive role for focal adhesion proteins in three-dimensional cell motility Nature Cell Biology. ,vol. 12, pp. 598- 604 ,(2010) , 10.1038/NCB2062