Mouse Meckel's cartilage chondrocytes evoke bone-like matrix and further transform into osteocyte-like cells in culture

摘要: Background We reported that when Meckel's cartilage was transplanted ectopically, chondrocytes transformed into osteocyte-like cells accompanying the extracellular calcified matrix. However, we could not determine whether were derived from host tissues or itself. Therefore, examined chondrocytes, which have a retrogressive ultimate fate, are capable of inducing observed calcification and further transform in culture. Methods Meckelian isolated enzymatically plated at low density grown α-MEM containing 10% FBS 37°C under 5% CO2 air for up to 4 weeks. Results Chondrocytes fibroblast-like early culture, but gradually polygonal typical showing metachromasia with toluidine blue staining. After an additional week large small round nodule formations. Small multiple-layered actively, showed more intense alkaline phosphatase (ALPase) activity. Immunostaining identified type II collagen matrix 2 weeks I osteocalcin later synthesized by cells. von Kossa's reaction extensive precipitation throughout flocculent materials. Ultrastructural analysis surrounded strongly resembled osteocytes. Conclusions The present study suggested can express phenotype vitro during synthesis bone-type marker proteins such as collagen. © 1996 Wiley-Liss, Inc.