A comprehensive evaluation and first molecular report of Theileria ovis infection in small ruminants in Saudi Arabia.

摘要: A total of 1000 clinically healthy small ruminants comprising 500 sheep and goats from five districts within Riyadh Province in Saudi Arabia were investigated by routine Giemsa staining for hematozoan parasites. Out these, 100 95 goat samples PCR using three pairs hemoprotozoan-specific primers. Based on microscopic examination, 33.2% 25.2% found infected with hemoprotozoan parasites, while detected infection 46% 33.7% goats. Extensive molecular characterization six species-specific primers revealed the dominance Theileria ovis, rather than any other species, which is recorded first time Arabia. Prevalence T. ovis was to be highest (32, 48%) followed AL-Kharj (31, 35%), Ad-Dawadimi 33%), AL-Majmaah (15, 27%), Rumah (17, 23%). The parasite prevalence 3 years age > 4 years ruminants, lowest < 1 year ruminants. No noticeable differences between male or female recorded. Partial sequencing 18S rRNA gene studied four new isolates ovis. Further pathogenicity clinical effects these highly needed. current results can helpful protecting improving livestock industry countries that depend a high number