Network analysis of KLF5 targets showing the potential oncogenic role of SNHG12 in colorectal cancer.

摘要: KLF5 is a member of the Kruppel-like factor, subfamily zinc finger proteins that are involved in cancers. functions as transcription factor and regulates diverse protein-coding genes (PCGs) colorectal cancer (CRC). However, long non-coding RNAs (lncRNAs) regulated by CRC currently unknown. In this study, we first designed computational pipeline to determine PCG lncRNA targets CRC. Then analyzed motif pattern binding regions for targets. The regulatory co-factors were then searched through bioinformatics analysis. We also constructed network annotated its functions. Finally, one targets, SNHG12, was selected further explore expression able identify 19 found motifs sites GC-enriched. Next, pinpointed factors AR HSF1 Then, analysis network, may be DNA replication, repair, cell cycle. Furthermore, cycle module, SNHG12 up-regulating verified lines tissues, associating it invasion distal metastasis. This indicates play critical part tumorigenesis progression. Additionally, down-regulated when knocked-down siRNA; strong correlation observed between levels KLF5, alluding their relationship. conclusion, significant