T-cell activation and early gene response in dogs.

摘要: T-cells play a crucial role in canine immunoregulation and defence against invading pathogens. Proliferation is fundamental to T-cell differentiation, homeostasis immune response. Initiation of proliferation following receptor mediated stimuli requires temporally programmed gene response that can be identified as immediate-early, mid- late phases. The immediate-early genes activation engage the cell cycle machinery promote subsequent events. Genes involved this dogs are yet identified. present study was undertaken characterise early improve understanding genetic mechanisms regulating function. Gene expression profiles were characterised using microarrays quantitative reverse transcription PCR (qRT-PCR), paired samples from eleven dogs. Significant functional annotation clusters stimulation with phytohemagluttinin (PHA) (5μg/ml), including Toll-like signaling pathway phosphorylation pathways. Using strict statistical criteria, 13 individual found differentially expressed, nine which have ontologies relate control. These included, prostaglandin-endoperoxide synthase 2 (PTGS2/COX2), growth 1 (EGR1), arrest DNA damage-inducible (GADD45B), phorbol-12-myristate-13-acetate-induced protein (PMAIP1), V-FOS FBJ murine osteosarcoma viral oncogene homolog (FOS), (EGR2), hemogen (HEMGN), polo-like kinase (PLK2) 3 (PLK3). Differential re-examined qRT-PCR, confirmed EGR1, EGR2, PMAIP1, PTGS2, FOS GADD45B significantly upregulated stimulated cells ALAS2 downregulated. PTGS2 EGR1 showed highest levels these Both regulation. This provides comprehensive analysis