Phytohaemagglutinin-induced proliferation of human T lymphocytes: differences between neonate and adults in accessory cell requirements.

摘要: The accessory cell requirements of human neonatal T lymphocytes were compared with those adult in lectin-induced polyclonal activation. It was found that purified Esh rosette positive not activated into a proliferative response by the lectin phytohaemagglutinin (PHA), phorbol ester (TPA) or conditioned medium containing growth factor activity (TCGF-CM). A to PHA obtained presence suitable (AC) such as plastic adherent, monocyte enriched population sIg lymphocyte population, both which shown cellular titrations be equally effective. Optimal responses could also obtained, absence cells, addition TPA TCGF-CM. Neonatal gave highly reproducible and this achieved effectively simple separation procedures involving further subfractionation responding Esh+ population. exquisite dependence these cells demonstrated investigation provides readily available model system for evaluation variables involved activation sensitive assay measuring activity.