Differences in the Induction of Induced Human CD4+ CD25+ FoxP3+ T-Regulatory Cells and CD3+ CD8+ CD28− T-Suppressor Cells Subset Phenotypes In Vitro: Comparison of Phorbol 12-Myristate 13-Acetate/Ionomycin and Phytohemagglutinin Stimulation

摘要: CD4(+) CD25(+) FoxP3(+) T-regulatory cells (Treg) and CD3(+) CD8(+) CD28(-) T-suppressor (Ts) were shown to have immunosuppressive function in vivo vitro. However, the vitro inducibility of Ts subsets is rather unclear. We investigated induction Treg peripheral blood mononuclear 5 healthy control individuals during stimulation with phorbol 12-myristate 13-acetate (PMA)/ionomycin or phytohemagglutinin (PHA). Phenotypes analyzed 0, 4, 8, 16, 24 hours after initiation cell culture using 4-color fluorescence flow-cytometry. Number CD127(-) increased PMA/ionomycin PHA (P < .01). coexpressed phenotypes interleukin (IL)-2(-), IL-10(+), and/or transforming growth factor (TGF)-β(+) (all P Interferon (IFN)-γ production was induced only by .01) but not = NS). IFN-γ-secreting detectable at 4 whereas IL-2(-), IL-10(+) TGF-β(+) required 16 stimulation. In contrast, inducible 24-hour IL-10 TGF-β polyclonal .01), proportion IL-2(-) remained stable period Similar Treg, detected conclude that remains They modify cytokine pattern indicating activation Ts. are IFN-γ- secreting form first line immunoregulatory T an initiated immune response followed Treg.