A simple, step-by-step dissection protocol for the rapid isolation of mouse dorsal root ganglia

摘要: The cell bodies of sensory neurons, which transmit information from the external environment to spinal cord, can be found at all levels column in paired structures called dorsal root ganglia (DRG). Rodent DRG neurons have long been studied laboratory improve understanding nerve development and function, instrumental determining mechanisms underlying pain neurodegeneration disorders peripheral nervous system. Here, we describe a simple, step-by-step protocol for swift isolation mouse DRG, enzymatically dissociated produce fully differentiated primary neuronal cultures, or processed downstream analyses, such as immunohistochemistry RNA profiling. After dissecting out column, base skull level femurs, it cut down mid-line cord meninges removed, before extracting detaching unwanted axons. This allows easy rapid with minimal practice dissection experience. process is both faster less technically challenging than situ after performing laminectomy. approach reduces time required collect thereby improving efficiency, permitting opportunity tissue deterioration, and, ultimately, increasing chances generating healthy cultures high quality, reproducible experiments using tissue.