Engineering bacteria for biogenic synthesis of chalcogenide nanomaterials.

摘要: Microbes naturally build nanoscale structures, including structures assembled from inorganic materials. Here, we combine the natural capabilities of microbes with engineered genetic control circuits to demonstrate ability biological synthesis chalcogenide nanomaterials in a heterologous host. We transferred reductase genes both Shewanella sp. ANA-3 and Salmonella enterica serovar Typhimurium into host (Escherichia coli) examined mechanisms that regulate properties biogenic nanomaterials. Expression arsenate thiosulfate E. coli resulted arsenic sulfide In addition processing starting materials via redox enzymes, cellular components also nucleated formation The shape nanomaterial was influenced by bacterial culture, synthetic strain producing nanospheres conditioned media or cultures wild-type nanofibres. diameter these nanofibres depended on context synthesis. These results potential for controlled combining wild tools biology.