Electron-nuclear double resonance of horseradish peroxidase compound I. Detection of the porphyrin pi-cation radical.
作者： J.E. Roberts , B.M. Hoffman , R. Rutter , L.P. Hager
DOI: 10.1016/S0021-9258(19)69747-4
关键词:
摘要: Electron-nuclear double resonance spectra of horseradish peroxidase Compound I exhibit resonances from 14N and beta-protons, most probably alpha-protons as well. The measurements prove that the oxyferryl iron this enzymic intermediate is spin-coupled to a porphyrin radical. Comparison experimentally obtained average pi-electron spin densities with theoretical predictions for 4-fold symmetric pi-cation radical supportive 2A2u assignment radical's electronic state.
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