EXAFS and XANES Studies of High-Valent Metal-Oxo Porphyrins Relevant to Horseradish Peroxidase

摘要: Horseradish peroxidase is a heme-containing enzyme that catalyzes the oxidation of phenolic substrates, utilizing hydrogen peroxide as ultimate electron acceptor. The resting form HRP contains high-spin ferric heme. During catalytic cycle, initially oxidized by two electrons to green species known Compound I (HRP-I). One reduction HRP-I yields red II (HRP-II), retains one oxidizing equivalent above enzyme. There substantial interest in understanding chemistry high-valent transition metal general and peroxidases particular because their importance oxygenation reactions. In this report we review our recent EXAFS XANES studies metalloporphyrins which are relevant horseradish compounds II.