A novel exonuclease (TaqMan) assay for rapid haptoglobin genotyping.
作者： Wilfried Renner , Renate Jahrbacher , Ernestine Marx-Neuhold , Simone Tischler , Barbara Zulus
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摘要: Background Haptoglobin is an acute-phase binding protein that scavenges free hemoglobin. The human haptoglobin gene (HP) polymorphic with two main alleles, allele 1 (Hp1) and 2 (Hp2). smaller Hp1 features no duplication consists of four exons, whereas the larger Hp2 allele, containing a 1.7 kb duplication, six fifth sixth being highly homologous to exons 3 4 Hp1. Methods We designed exonuclease (TaqMan) assay targeting single nucleotide differences between regions Hp2. contained one probe specifically site in intron Hp2, another equally 6 Results Measurement post-PCR fluorescence allowed unambiguous discrimination HP genotypes. Comparison genotypes obtained by method based upon allele-specific primers yielded fully corresponding results. Conclusions new genotyping fast, reliable, does not require real-time instruments may be especially useful for high-throughput genotyping.
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