Tri-ortho-cresyl Phosphate Induces Autophagy of Mouse Ovarian Granulosa Cells
作者： Jinglei Wang , Wenli Ruan , Boshu Huang , Shuxin Shao , Dan Yang
DOI: 10.1530/REP-18-0456
关键词:
摘要: Tri-ortho-cresyl phosphate (TOCP) has been widely used as plasticizers, plastic softeners and flame-retardants in industry reported to have male reproductive toxicology. However, it is still unknown whether TOCP affects the female system its underlying mechanism. In present study, we found that exposure significantly decreased ovarian coefficient, caused disintegration depletion of granulosa cells ovary tissue inhibited level serum estradiol (E2). markedly increased both LC3-II ratio LC3-II/LC3-I well autophagy proteins ATG5 Beclin1 tissue, implying could induce tissue. To further investigate potential mechanism, primary were isolated vitro treated with 0-0.5 mM for 48 h. We showed number viable mouse without affecting cell cycle apoptosis cells. Intriguingly, treatment Beclin1. Furthermore, transmission electron microscopy (TEM) autophagic vesicles cytoplasm TOCP-treated cells, indicating Taken together, reduces induces apoptosis.
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Maurice I. Smith, E. Elvove, Pharmacological and Chemical Studies of the Cause of so-called Ginger Paralysis. A Preliminary Report. Public Health Reports. ,vol. 45, pp. 1703- 1716 ,(1930) , 10.2307/4579730
Craig R. Brinkerhoff, Raghubir P. Sharma, Denis R. Bourcier, The effects of tri-o-tolyl phosphate (TOTP) on the immune system of mice Ecotoxicology and Environmental Safety. ,vol. 5, pp. 368- 376 ,(1981) , 10.1016/0147-6513(81)90010-5
Yukiko Kabeya, Noboru Mizushima, Takashi Ueno, Akitsugu Yamamoto, Takayoshi Kirisako, Takeshi Noda, Eiki Kominami, Yoshinori Ohsumi, Tamotsu Yoshimori, LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing The EMBO Journal. ,vol. 19, pp. 5720- 5728 ,(2000) , 10.1093/EMBOJ/19.21.5720
Guilherme L. Emerick, Marion Ehrich, Bernard S. Jortner, Regina V. Oliveira, Georgino H. DeOliveira, Biochemical, histopathological and clinical evaluation of delayed effects caused by methamidophos isoforms and TOCP in hens: Ameliorative effects using control of calcium homeostasis Toxicology. ,vol. 302, pp. 88- 95 ,(2012) , 10.1016/J.TOX.2012.08.002
M.Y. Yang, J.E. Fortune, Vascular endothelial growth factor stimulates the primary to secondary follicle transition in bovine follicles in vitro. Molecular Reproduction and Development. ,vol. 74, pp. 1095- 1104 ,(2007) , 10.1002/MRD.20633
Stephen G. Somkuti, Daniel M. Lapadula, Robert E. Chapin, James C. Lamb, Mohamed B. Abou-Donia, Testicular toxicity following oral administration of tri-O-cresyl phosphate (TOCP) in roosters Toxicology Letters. ,vol. 37, pp. 279- 290 ,(1987) , 10.1016/0378-4274(87)90143-3
Margo l. Hennet, Catherine M. H. Combelles, The antral follicle: a microenvironment for oocyte differentiation. The International Journal of Developmental Biology. ,vol. 56, pp. 819- 831 ,(2012) , 10.1387/IJDB.120133CC
Jia-Xiang Chen, Ying-Jian Sun, Pan Wang, Ding-Xin Long, Wei Li, Li Li, Yi-Jun Wu, None, Induction of autophagy by TOCP in differentiated human neuroblastoma cells lead to degradation of cytoskeletal components and inhibition of neurite outgrowth Toxicology. ,vol. 310, pp. 92- 97 ,(2013) , 10.1016/J.TOX.2013.05.012
Stephen G Somkuti, Daniel M Lapadula, Robert E Chapin, Mohamed B Abou-Donia, Light and electron microscopic evidence of tri-o-cresyl phosphate (TOCP)-mediated testicular toxicity in Fischer 344 rats Toxicology and Applied Pharmacology. ,vol. 107, pp. 35- 46 ,(1991) , 10.1016/0041-008X(91)90328-C
Li-Ping Zhang, Qing-Shan Wang, Xin Guo, Ying-Jian Zhu, Gui-Zhen Zhou, Ke-Qin Xie, Time-dependent changes of lipid peroxidation and antioxidative status in nerve tissues of hens treated with tri-ortho-cresyl phosphate (TOCP). Toxicology. ,vol. 239, pp. 45- 52 ,(2007) , 10.1016/J.TOX.2007.06.091