Blueberry Peel Extracts Inhibit Adipogenesis in 3T3-L1 Cells and Reduce High-Fat Diet-Induced Obesity

摘要: This study examined the anti-obesity effect and mechanism of action blueberry peel extracts (BPE) in 3T3-L1 cells high-fat diet (HFD)-induced obese rats. The levels lipid accumulation were measured, along with changes expression genes proteins associated adipocyte differentiation cells. Evidenced by Oil-red O staining triglyceride assay, BPE dose-dependently inhibited at concentrations 0, 50, 200 µg/ml. decreased key regulator C/EBPβ, as well C/EBPα PPARγ genes, during preadipocytes into adipocytes. Moreover, down-regulated adipocyte-specific such aP2 FAS compared control specific mediating effects BP revealed that insulin-stimulated phosphorylation Akt was strongly decreased, its downstream substrate, phospho-GSK3β, downregulated treatment Together, these data indicated exerted anti-adipogenic activity inhibiting C/EBPβ signaling pathway Next, we investigated whether attenuated HFD-induced obesity Oral administration reduced body weight gain significantly without affecting food intake. epididymal or perirenal adipose tissue weights lower rats on an HFD plus Total cholesterol fed modestly reduced, HDL-cholesterol level increased BP-fed those HFD-fed Taken together, results demonstrated inhibitory adipogenesis through down-regulation C/EBPα, reduction phospho-Akt adipogenic factor fat animal model obesity.