Enzymatic modification of hydroxyethylcellulose by transgalactosylation with β-galactosidases
作者： Jun Li , H.N. Cheng , Robert G. Nickol , Peng George Wang
DOI: 10.1016/S0008-6215(99)00041-5
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摘要: Abstract β-galactosidases from A. oryzae and a thermophilic CLONEZYME™ glycosidase library were used to catalyze the transfer of β- d -galactopyranosyl moiety lactose hydroxyl groups hydroxyethylcellulose (HEC) in sodium acetate buffer. The degree substitution was quantified by using galactose oxidase enzymatic assays. Depolymerization also observed course transglycosylation reactions.
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