Purification and characterization of recombinant single-chain urokinase produced in Escherichia coli.
作者： Marjorie E. Winkler , Michael Blaber
DOI: 10.1021/BI00362A008
关键词:
摘要: Recombinant single-chain urokinase (rUK1) has been purified from Escherichia coli. The purification utilizes a refractile body purification, followed by batch DE-52 cellulose extraction, hydroxylapatite chromatography, and S-200 chromatography. Two-chain rUK (rUK2) is separated rUK1 on benzamidine--Sepharose. eliminates proteases early in the procedure so will not be cleaved to rUK2. characterized amino-terminal analysis as well carboxy-terminal after cleavage plasmin.
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