Quantification of major classes of Xenopus phospholipids by high performance liquid chromatography with evaporative light scattering detection.

摘要: Lipid signaling has become a major research area of cell biology and there is need for methods that accu- rately easily measure substrates products lipases involved in signaling. In this report, we provide new methodology separation more than 10 lipids one chromatographic run by high pressure liquid chromatogra- phy (HPLC) detection with an evaporative light scatter- ing detector (ELSD). There no significant loss sphingo- myelin large baseline change, peak obscures another, acidic phospholipids are cleanly separated. We have optimized the procedure two-pump HPLC, in- expensive silica column without use heater jacket low grade nitrogen. An application separates from Xenopus laevis cells. These cells commonly used study various lipid signal- paths division, fertilization, after expression exogenous membrane receptors. —Stith, B. J., J. Hall, P. Ayres, L. Waggoner, D. Moore, W. A. Shaw. Quantifi- cation classes performance chromatography scattering . Res. 2000. 41: 1448-1454.