Molecular and biochemical approaches to determining what bacteria are doing in vivo.

摘要: Although the results of experiments carried out to date have not given unambiguous answers question which polysaccharides are being utilized byBacterioides in colon, nonetheless encourage us think that it may be possible develop methods for answering this and related questions furture. Given complexity vivo environmental impossibility knowing, much less controlling, all parameters, will probably necessary use more than one approach a particular assume if anseers obtained by different approaches consistent, they correct. For example, general conclusions from testing performance mutants germfree mouse model agreement with those arising direct biochemical analysis human feces. Also, these help guide design specific antibody probes because indicate most likely important. A bootstrap type each successive set produces information makes probe only sense when dealing metabolically versatile organisms highly complex ecosystem.