Stimulation of pp60c-src kinase activity in FDC-P1 cells by polyoma middle-T antigen and hematopoietic growth factors.

摘要: Either of two hematopoietic growth factors, GM-CSF or IL-3, are required for the bone marrow-derived FDC-P1 cell line. These factors induce cellular tyrosine-specific protein kinases when added to resting cells. The receptors these have not been unambiguously shown contain a kinase domain. To determine whether src-related kinases, in particular pp60-c-src, regulated by cells were transfected with plasmids carrying polyoma middle-T antigen, potent activator pp60c-src. Middle-T-expressing showed reduced requirement and IL-3 selected clones grew absence factors. Middle-T formed complex pp60c-src stimulated its vitro activity 20-50 fold. was further increased if middle-T-expressing, factor-independent treated IL-3.