Complete nucleotide sequence of an isolate of Euphorbia mosaic virus that infects Euphorbia heterophylla and Wissadula amplissima in Jamaica

摘要: Begomoviruses (family Geminiviridae) are whitefly-transmitted viral phytopathogens that devastate many cash and basic food crops worldwide [6]. Euphorbia heterophylla Wissadula amplissima widely distributed throughout Jamaica oftentimes exhibit yellow mosaic leaf curl symptoms. The agent causes of E. in has not been identified; however, partial sequences isolated from symptomatic W. suggest it is infected by three distinct begomoviruses, one which was tentatively named golden St Elizabeth virus (WGMSEV) [1, 5]. In Mexico, the begomovirus (EuMV) [3]. Here, we report WGMSEV actually an isolate EuMV infects both Jamaica, this genome features distinguishes previously reported isolates. In 2006, sample W96 [1], were isolated, exhibiting collected Dunder Hill parish Elizabeth. Total DNA extracted tissues these plants [4] used as template PCR. available for enabled design primers to amplify complete DNA-A DNA-B (Supplementary data, Table 1). Amplicons cloned sequenced at Macrogen (South Korea). Basic Local Alignment Search Tool (BLAST, http://www.ncbi.nlm.nih.gov/) sequence similarity searches, whilst DNAStar (DNAStar Inc., Madison, WI, USA) alignments phylogenetic inferences. Full-length clones pEAFL15 ({"type":"entrez-nucleotide","attrs":{"text":"FJ407052","term_id":"212420343","term_text":"FJ407052"}}FJ407052) pW96AFL15 ({"type":"entrez-nucleotide","attrs":{"text":"DQ395342","term_id":"148628009","term_text":"DQ395342"}}DQ395342) W96, respectively. These 2,609 nt 99% identical, suggesting they isolates a single begomovirus. Clone representative subsequent analyses. [1] 97% identical corresponding region pW96AFL15, could represent WGMSEV. BLAST searches showed most similar Western Hemisphere begomoviruses shared highest identities with EuMV. 96% A strain Jurabo, Puerto Rico, 1991 (EuMV-A[PR:Jur:91]) B Jalasco, 2005 (EuMV-B[MX:Jal:05]). Additionally, 92% Yucatan, 2004 (EuMVA[MX:Yuc:04]). sharing greater than 89% identity constitute species [2], tentative therefore renamed EuMV-[Jamaica:StElizabeth:2006], abbreviated EuMV-[JM:SE:06]. full-length clone pW96BFL1 ({"type":"entrez-nucleotide","attrs":{"text":"EU740969","term_id":"190576829","term_text":"EU740969"}}EU740969), 2571 likely EuMV-[JM:SE:06], 165-nt CR DNA-A. 83%, EuMVA[MX:Yuc:04]. Phylogenetic analyses placed EuMV-[JM:SE:06] squash cluster, EuMV-[MX:Yuc:04] [3]. Comparison all four revealed EuMV-B[MX:Jal:05] highly congruent diverge EuMV-A size (Fig. 1a). only, differ mostly their long non-coding left right hypervariable intergenic regions 1b). Comparison different strains within other countries \89% same country have [90% 2). 83% between EuMV-A[MX:Yuc:04] accord lower countries. Fig. 1 Illustration divergence isolates. b DNA-B. RIR region, LIR region The experimental host range investigated biolistic inoculation using tandem repeats its components Fig. 2), Capsicum annum, Lycopersicon esculentum, N. benthamiana Phaseolous vulgaris, peppers, serves natural reservoir can be disseminated maintain facilitate adaptation crops.