Two new bipartite begomoviruses infecting Wissadula amplissima in Jamaica.

摘要: Begomoviruses are whitefly transmitted, single-stranded DNA viruses that infect dicotyledons (Stanley et al. , 2005). In Jamaica, the weed Wissadula amplissima is a host of begomovirus golden mosaic virus (WGMV)(Roye 1997). Total was extracted from two W. plants (W96 and W132) showing symptoms, collected in November 2004 February 2005 respectively. DNA-A DNA-B amplicons 1·4 kb were generated these samples PCR using primer pairs PAC1v1978/PAV1c715 PBC1v2039/PBV1c800 for These fragments cloned sequenced (Rojas 1993). Analysis intergenic region (IR) sequence, revealed DNAA clones sample 96 (W96AT W96BT respectively) cognate components bipartite with 94% sequence identity. Clone pW96AT (1385 nt; DQ395342) encompasses IR 5 ′ ends replication-associated coat protein genes, showed highest nucleotide identity (81%) to Tomato mild yellow leaf curl Aragua (AY927277). pW96BT, consisting 845 nt encompassing part nuclear shuttle ( NSP ) gene IR, (50%) Macroptilium -[Jamaica: 2] (AF098939). A less than 89% reported geminiviruses suggests this geminivirus previously unreported has been tentatively named St. Elizabeth (WGMSEV). The IRs 132 (W132AT W132BT) show identity, suggesting they could form an infectious unit. W132AT (1384 nt, DQ395343) most closely related (77%) WGMV-[Jamaica:1] Jatropha (JMV; AF324410), whilst W132BT (DQ395344) shows 62% JMV (AF324411) over 1488 movement genes IR. This provisionally Thomas (WGMSTV). WGMSTV WGMSEV 55% 37% first report WGMSTV.