Non-competitive time-resolved immunofluorometric assays for determination of human insulin-like growth factor I and II.

摘要: We present sensitive non-competitive time-resolved immunofluorometric assays (TR-IFMAs) for IGF-I and IGF-II based on monoclonal antibodies. Assays were performed in microtest-plate wells: the first antibodies immobilized solid matrix, second labelled with chelate derivative of Europium (Eu3+). The obtained specificities sensitivities high: cross-reactivity heterologous assay was below 0.0002%. detection limits 0.0025 micrograms/l 0.010 assay, respectively. operating range included upwards: 2.5 (IGF-I) 10.0 (IGF-II). This implies that all clinically relevant serum concentrations could be measured one final dilution (1:1066 1:2132 IGF-II) after acid ethanol extraction. high sample buffer made further neutralization or evaporation extracts unnecessary. Interassay variation 10%.