gp130 Transducing Receptor Cross-linking Is Sufficient to Induce Interleukin-6 Type Responses

摘要: Abstract gp130 transducing receptor is involved in the formation of high affinity receptors for cytokines interleukin-6 (IL-6) family. Recruitment by IL-6 associated to its leads dimerization component. In present study we did characterize B-S12 monoclonal antibody raised against and able elicit type biological activities. triggered strongly proliferation TF1 XG1 hematopoietic cell lines was increase synthesis acute phase proteins HepG2 hepatoma line. also behaved as a synergistic factor with granulocyte-macrophage colony-stimulating both differentiation CD34-positive progenitors. By using symmetric enzyme-linked immunosorbent assay, allowing detection dimeric forms soluble gp130, found that addition led dimerization. Analysis tyrosine phosphorylation events Jak kinase family members revealed quickly induced neural derived line, Jak1 Jak2 were recruited. conclusion, show cross-linking sufficient generate functional responses hematopoietic, neural, hepatic cells.