Monitoring of the refolding process for immobilized firefly luciferase with a biosensor based on surface plasmon resonance.

摘要: In order to examine the possibility of use a surface plasmon resonance (SPR) sensor for real-time monitoring process refolding immobilized proteins, firefly luciferase on carboxymethyldextran matrix layer was analyzed. The SPR signal decreased after unfolding induced by GdnCl and increased gradually in buffer, while there no change reference lacking protein. decrease consistent with difference between refractive indices native unfolded protein solutions. effects blocking excess NHS-groups yield were examined means an sensor. results those obtained enzymatic activity assay, indicating that changes reflected conformational Hence, biosensor might be used proteins as novel tool optimization conditions. This is first demonstration reflect upon refolding.