Continuous flow immunosensor for highly selective and real-time detection of sub-ppb levels of 2-hydroxybiphenyl by using surface plasmon resonance imaging

摘要: Abstract A biosensor based on surface plasmon resonance (SPR) is developed for the detection of 2-hydroxybiphenyl (HBP). monoclonal antibody against HBP (abbreviated hereafter as HBP-mAb) and used by competitive SPR-based immunoassay enzyme linked immunosorbent assay (ELISA) methods. novel HBP-hapten compound, HBP–bovine serum albumin conjugate (HBP–BSA), derived binding several units with BSA an aliphatic chain spacer in development functionalization immunoprobes. HBP–BSA to Au SPR sensor chip undergoes inhibitive immunoreaction HBP-mAb presence free HBP. The provides a rapid determination (response time: ∼20 min) concentration range 0.1–1000 ppb (ng/ml). Regeneration gained treating antibody-anchored pepsin solution (100 ppm (μg/ml); pH 2.0) few minutes. reusable more than 20 cycles average loss 0.35% reactivity per regeneration step. determined low 0.1 3 ppb using ELISA measurements, respectively. from interference coexisting benzo[ ]pyrene (BaP), 2,4-dichlorophenoxyacetic acid (2,4-D) benz[ ]anthracene; angle shift obtained flow almost same irrespective or absence these carcinogenic polycyclic aromatic hydrocarbons together. proved be applicable simultaneous BaP parallel another BaP.