DNA-protein cross-linking and DNA interstrand cross-linking by haloethylnitrosoureas in L1210 cells.

摘要: Abstract Bifunctional alkylating agents are known to produce cross-links between DNA and protein paired strands. The possibility of discriminating these two classes in L1210 cells treated with haloethylnitrosoureas or nitrogen mustard was explored the alkaline elution technique. Two were demonstrated, based on sensitivity proteinase K; proteinase-sensitive appear be DNA-protein cross-links, proteinase-resistant class may include interstrand cross-links. Proteinase-sensitive form more rapidly than do chloroethylnitrosoureas, perhaps because can chloroethylate sulfhydryl amino groups followed by rapid reaction chloroethylated DNA. Although both types produced disappeared repaired after 24 hr, removal chloroethylnitrosoureas either did not occur incomplete hr. In addition exhibited strand breaks; a method is suggested for estimating apparent frequencies breaks