Capnellene, a natural marine compound derived from soft coral, attenuates chronic constriction injury-induced neuropathic pain in rats.
作者： Yen-Hsuan Jean , Wu-Fu Chen , Chun-Sung Sung , Chang-Yih Duh , Shi-Ying Huang
DOI: 10.1111/J.1476-5381.2009.00323.X
关键词:
摘要: Background and purpose: Natural compounds obtained from marine organisms have received considerable attention as potential sources of novel drugs for treatment human inflammatory diseases. Capnellene, isolated the soft coral Capnella imbricate, 4,4,6a-trimethyl-3-methylene-decahydro-cyclopenta[]pentalene-2,3a-diol (GB9) exhibited anti-inflammatory actions on activated macrophages in vitro. Here we assessed anti-neuroinflammatory properties GB9 its acetylated derivative, acetic acid 3a-hydroxy-4,4,6a-trimethyl-3-methylene-decahydro-cyclopenta[]pentalen-2-yl ester (GB10). Experimental approach: Effects or GB10 expression inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) interferon-γ (IFN-γ)-stimulated mouse microglial BV2 cells were measured by Western blot. The vivo effects these examined chronic constriction injury (CCI) rat model neuropathic pain, measuring thermal hyperalgesia, activation COX-2 protein lumbar spinal cord, immunohistochemistry. Key results: In cells, inhibited iNOS COX-2, stimulated IFN-γ. Intrathecal administration CCI-induced nociceptive sensitization hyperalgesia a dose-dependent manner. Intraperitoneal injection also up-regulation dorsal horn cord ipsilateral to injury. Conclusion implications: Taken together, data indicate that marine-derived capnellenes, GB10, had anti-nociceptive IFN-γ-stimulated rats respectively. Therefore, capnellene may serve useful lead compound search new therapeutic agents neuroinflammatory
sci-hub.se 参考文章(78)
Dan Levy, Paul Kubes, Douglas W. Zochodne, Delayed peripheral nerve degeneration, regeneration, and pain in mice lacking inducible nitric oxide synthase. Journal of Neuropathology and Experimental Neurology. ,vol. 60, pp. 411- 421 ,(2001) , 10.1093/JNEN/60.5.411
Y.M. Sheikh, G. Singy, M. Kaisin, H. Eggert, Carl Djerassi, B. Tursch, D. Daloze, J.C. Braekman, Terpenoids-LXXI. Chemical studies of marine invertebrates-XIV. Four representatives of a novel sesquiterpene class-the capnellane skeleton Tetrahedron. ,vol. 32, pp. 1171- 1178 ,(1976) , 10.1016/0040-4020(76)85041-7
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
Fei-Yu Zhang, You Wan, Zhen-Kang Zhang, Alan R. Light, Kai-Yuan Fu, Peripheral formalin injection induces long-lasting increases in cyclooxygenase 1 expression by microglia in the spinal cord. The Journal of Pain. ,vol. 8, pp. 110- 117 ,(2007) , 10.1016/J.JPAIN.2006.06.006
Russell K. Portenoy, Kathleen M. Foley, Charles E. Inturrisi, The nature of opioid responsiveness and its implications for neuropathic pain: new hypotheses derived from studies of opioid infusions Pain. ,vol. 43, pp. 273- 286 ,(1990) , 10.1016/0304-3959(90)90025-9
D.Michele Basso, Michael S. Beattie, Jacqueline C. Bresnahan, Graded Histological and Locomotor Outcomes after Spinal Cord Contusion Using the NYU Weight-Drop Device versus Transection Experimental Neurology. ,vol. 139, pp. 244- 256 ,(1996) , 10.1006/EXNR.1996.0098
J. R. Zurek, R. Nadeson, C. S. Goodchild, Spinal and supraspinal components of opioid antinociception in streptozotocin induced diabetic neuropathy in rats Pain. ,vol. 90, pp. 57- 63 ,(2001) , 10.1016/S0304-3959(00)00386-9
Bryan C Hains, Stephen G Waxman, Activated Microglia Contribute to the Maintenance of Chronic Pain after Spinal Cord Injury The Journal of Neuroscience. ,vol. 26, pp. 4308- 4317 ,(2006) , 10.1523/JNEUROSCI.0003-06.2006
M. Svensson, P. Eriksson, J.K.E. Persson, C. Molander, J. Arvidsson, H. Aldskogius, The response of central glia to peripheral nerve injury Brain Research Bulletin. ,vol. 30, pp. 499- 506 ,(1993) , 10.1016/0361-9230(93)90284-I
S.T. Meller, C. Dykstra, D. Grzybycki, S. Murphy, G.F. Gebhart, The possible role of glia in nociceptive processing and hyperalgesia in the spinal cord of the rat. Neuropharmacology. ,vol. 33, pp. 1471- 1478 ,(1994) , 10.1016/0028-3908(94)90051-5