miR-137 regulates epithelial-mesenchymal transition in gastrointestinal stromal tumor

摘要: Activation of the epithelial-to-mesenchymal transition (EMT) endows extraordinary invasive capability cancer cells and causes treatment failure metastasis in gastrointestinal stromal tumor (GIST); however, molecular mechanisms governing GIST invasion remain largely unknown. MicroRNAs (miRNAs) have been shown to play critical roles cell motility invasion, which promotes us study biological functions miR-137 EMT GIST. We found that was dramatically downregulated clinical specimen Using an silico analysis approach, Twist1, a key regulator gene EMT, has identified as target miR-137. Quantitative RT-PCT western blot were used confirm directly targeted on Twist1 repressed expression GIST-H1 human line. Further, increase E-cadherin cytokeratin, but suppress N-cadherin vimentin. In vitro experiments enhanced epithelial morphology, decreased migration, activated G1 cycle arrest, induced apoptosis. These results suggest novel mechanism regulates inhibits migration via downregulation. Therefore, may function anti-migration anti-metastasis our provides potential approach for developing miR-137-based therapeutic strategy